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Effect of temperature and viscosity of sieving medium on electrophoretic behavior of sodium dodecyl sulfate‐proteins on capillary electrophoresis in presence of pullulan
Author(s) -
Nakatani Manabu,
Shibukawa Akimasa,
Nakagawa Terumichi
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170707
Subject(s) - sodium dodecyl sulfate , chromatography , pullulan , electrophoresis , capillary electrophoresis , chemistry , viscosity , sodium , biochemistry , materials science , organic chemistry , polysaccharide , composite material
Pullulan was used as a sieving medium in high‐performance capillary gel electrophoresis to study the effect of temperature and viscosity on electrophoretic behavior of sodium dodecyl sulfate (SDS)‐proteins with molecular masses in the range of 14 400–116 000 Da. The migration time decreased with increasing capillary temperature. Equations were derived relating mobility of SDS‐proteins to capillary temperature and viscosity of pullulan solution. Linear relationships were obtained from experiments between logarithm of mobility and reciprocal temperature and between double logarithmic values of mobility and viscosity of pullulan solution. The experimental results agree well with the equations.