Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose | Zendy

Schwinefus Jeffrey James | Zendy; Wang ShauChun | Zendy; Hammond Richard W. | Zendy; Morris Michael D. | Zendy

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Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose

Author(s) -

Schwinefus Jeffrey James,

Wang ShauChun,

Hammond Richard W.,

Morris Michael D.

Publication year - 1996

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150170621

Subject(s) - förster resonance energy transfer , fluorescence , dna , electrophoresis , chemistry , energy transfer , fluorescence microscope , biophysics , analytical chemistry (journal) , chemical physics , chromatography , optics , biochemistry , biology , physics

Fluoresence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.

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