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Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose
Author(s) -
Schwinefus Jeffrey James,
Wang ShauChun,
Hammond Richard W.,
Morris Michael D.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170621
Subject(s) - förster resonance energy transfer , fluorescence , dna , electrophoresis , chemistry , energy transfer , fluorescence microscope , biophysics , analytical chemistry (journal) , chemical physics , chromatography , optics , biochemistry , biology , physics
Fluoresence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.