Premium
The limiting mobility of DNA sequencing fragments for both cross‐linked and noncross‐linked polymers in capillary electrophoresis: DNA sequencing at 1200 V cm −1
Author(s) -
Yan Juying,
Best Norine,
Zhang Jian Zhong,
Ren Hongji,
Jiang Rong,
Hou Joan,
Dovichi Norman J.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170611
Subject(s) - reptation , electric field , dna , capillary electrophoresis , electrophoresis , analytical chemistry (journal) , dna sequencing , field (mathematics) , inverse , limiting , chemistry , polymer , chromatography , physics , biochemistry , geometry , mathematics , organic chemistry , quantum mechanics , pure mathematics , mechanical engineering , engineering
The mobility of DNA sequencing fragments was measured in Long‐Ranger gels at an electric field ranging from 200 to 1200 V cm −1 and in noncross‐linked polyacrylamide at electric fields ranging from 100 to 300 V cm −1 . In both cases, N *, the fragment length that denotes the onset of biased reptation with orientation, is inversely proportional to electric field. The inverse dependence of N * is inconsistent with the original biased reptation model but is consistent with modern models of DNA migration. While separation speed increases dramatically with electric field, the number of bases determined in a separation decreases in proportion to field strength. We present a DNA sequencing run at an electric field of 1200 V cm −1 . Roughly 200 bases of sequence are determined in 3.5 min.