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Alpha‐2‐HS‐glycoprotein (AHSG) polymorphism in semen and saliva
Author(s) -
Yasuda Toshihiro,
Takeshita Haruo,
Tsubota Etsuko,
Sawazaki Kazumi,
Iida Reiko,
Nadano Daita,
Kishi Koichiro
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170429
Subject(s) - saliva , isoelectric focusing , semen , chemistry , chromatography , biology , biochemistry , genetics , enzyme
Polymorphism of alpha‐2‐HS‐glycoprotein (AHSG) was demonstrated in human semen and whole saliva samples by thin‐layer polyacrylamide gel isoelectric focusing (IEF) and immunoblotting. Although the seminal AHSG IEF patterns were found to differ from those of plasma AHSG from the corresponding donors, incorporation of Nonidet P–40 into the IEF gel (pH 4.2–4.9) enabled us to phenotype seminal AHSG correctly. Salivary AHSG, however, exhibited IEF patterns similar to those of the corresponding plasma AHSG. By treating the samples with neuraminidase, it was possible to determine the AHSG types using 2–5 μL semen and 50–100 μL whole saliva samples. The AHSG types determined separately in 47 sets of semen, whole saliva, urine and plasma samples from the same donors correlated perfectly with each other. AHSG typing could, therefore, provide an additional discriminant characteristic in the forensic examination of semen and saliva samples.