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The role of pH and membrane porosity in preparative electrophoresis
Author(s) -
Corthals Garry L.,
Margolis Joel,
Williams Keith L.,
Gooley Andrew A.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170425
Subject(s) - isoelectric point , fractionation , chromatography , electrophoresis , chemistry , ovalbumin , isoelectric focusing , free flow electrophoresis , gel electrophoresis , porosity , bovine serum albumin , analytical chemistry (journal) , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , biology , enzyme , organic chemistry , immune system , immunology
The Gradiflow is a preparative electrophoresis apparatus, allowing fractionation based on a combination of size and charge of proteins in their native (unreduced) form. The prepative fractionation of two proteins of similar size and isoelectric point is demonstrated using the Gradiflow. A separation membrance of appropriate pore size was chosen and then fractionation was “fine tuned” by selecting an appropriate buffer pH to accentuate charge differences between the proteins of interest. Complete separation of mg quantities of bovine serum albumin and ovalbumin was achieved within 40 min Presented at the Second Annual Meeting of the Australian Electrophoresis Society, Sydney, April 29–30, 1995. .