Premium
Capillary zone electrophoresis in agarose gels using absorption imaging detection
Author(s) -
Palm Anders,
Lindh Curt,
Hjertén Stellan,
Pawliszyn Janusz
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170424
Subject(s) - capillary electrophoresis , electrophoresis , agarose , chromatography , chemistry , capillary action , substrate (aquarium) , materials science , analytical chemistry (journal) , oceanography , composite material , geology
A simple home‐built electrophoretic unit and a commercially available chargecoupled device (CCD) camera with image acquisition and analysis software were used to study the separation process in zone electrophoresis experiments in 4 cm long, round capillaries (inside diameter 0.2 mm). Several capillaries could be investigated simultaneously. The absorption imaging system was used not only to follow the course of the separation but also to study the interaction between biologically active substances (proteins, detergents, enzymes and substrate). Since the system allows visual on‐line observation of the separation one can rapidly decide when the analysis is finished, which often shortens the analysis time. The electrophoresis method presented is suitable also for preparative runs, since direct visualizations of a solute zone allows it to be excised and then used for further studies.