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Molecular analysis of Salmonella enterica serotype Dublin: Building bridges between population genetic and molecular epidemiological studies
Author(s) -
Platt David J.,
Browning Lynda M.,
Candlish Denise
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170407
Subject(s) - plasmid , biology , genetics , restriction enzyme , population , agarose gel electrophoresis , salmonella enterica , serotype , molecular epidemiology , dna , microbiology and biotechnology , genotype , escherichia coli , gene , demography , sociology
Population genetic studies of Salmonella enterica serotype Dublin using multilocus enzyme electrophoresis have recognised two dominant clones termed Du1 and Du3. The characterisation of plasmids in Dublin suggests greater strain diversity. The application of restriction enzyme fragmentation pattern (REFP) analysis of genomic DNA using Sau 3A and Hinc II together with plasmid subtractive analysis can resolve anomalies in earlier comparisons. Twenty‐six isolates were selected for inclusion in the study. All had been previously characterised with respect to their plasmids, and were isolated from the USA, Canada and five European countries. On the basis of plasmid profiles, 17 were predicted to correspond with Du1 and Du3. Sau 3A digestion generated two distinct REFPs (A and B) of < 70% similarity, which corresponded with Du1 and Du3. After the contribution of plasmid‐derived bands was subtracted, two variants of A (A1 and A2) and four of B (B1, B2, B3 and B4) were recognised. Seventeen were concordant with predictions from population genetic studies. Nine that could not be predicted on the basis of atypical plasmid profiles all showed REFP A1 (Du1) and were consistent with the incursion of additional plasmids or plasmid cointegration. REFPs from Hinc II digests generally corroborated Sau 3A data but showed greater overall similarity between the strains and more influence from plasmid DNA Presented at the British Electrophoresis Society Meeting, Maidstone Kent, UK, July 13–14, 1995. .