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Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation
Author(s) -
Nair T. Murlidharan,
Madhusudan K.,
Nagaraja V.,
Kulkarni B. D.,
Majumdar H. K.,
Singh Rajan
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170403
Subject(s) - permutation (music) , locus (genetics) , curvature , dna , circular dna , genetics , fragment (logic) , biology , combinatorics , physics , mathematics , geometry , gene , algorithm , acoustics , genome
Using the theoretical model for DNA curvature, we analyzed a set of fragments with a curved insert located in circular permutation. The theoretical permutation analysis of each of the cyclically located fragments reveals the presence of a shifting molecular bend locus. The delineation of the molecular bend locus associated with the fragments obtained by a second permutation helps in providing an explanation for the differential mobility behavior of the fragments.