Premium
Two‐dimensional electrophoretic patterns of acute‐phase human serum proteins in the course of bacterial and viral diseases
Author(s) -
Bini Luca,
Magi Barbara,
Marzocchi Barbara,
Cellesi Carla,
Berti Brunilde,
Raggiaschi Roberto,
Rossolini Aldo,
Pallini Vitaliano
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170333
Subject(s) - haptoglobin , acute phase protein , blood proteins , isoelectric focusing , electrophoresis , serum amyloid a , transthyretin , orosomucoid , isoelectric point , densitometry , glycoprotein , antiserum , biology , chemistry , microbiology and biotechnology , biochemistry , immunology , antibody , medicine , inflammation , enzyme , endocrinology
Acute‐phase serum proteins were analyzed by two‐dimensional electrophoresis with isoelectric focusing in 3‐10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS‐2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of α1‐antitrypsin, leucinerich glycoprotein, haptoglobin, serum retinol‐binding protein and transthyretin were quantitated by densitometry of silver‐stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As for the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.