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The yeast SWISS‐2DPAGE database
Author(s) -
Sanchez JeanCharles,
Golaz Olivier,
Frutiger Séverine,
Schaller Dominique,
Appel Ron D.,
Bairoch Amos,
Hughes Graham J.,
Hochstrasser Denis F.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170326
Subject(s) - yeast , database , computational biology , biology , computer science , genetics
The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein‐based technologies such as two‐dimensional gel eletrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS‐2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS‐2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.