Phenotyping of esterase D and acid phosphatase by rapid isoeletric focusing on PhastSystem

A rapid isoelectric focusing method on PhastSystem was applied to investigate the distribution of esterase D (ESD) and acid phosphatase (ACP) phenotypes in 152 and 112 unrelated healthy donors, respectively, from the Han population in Beijing. Laboratory‐made gels with a pH range of 5.0–7.0 and 5.0–8.0 were used for analysis of ESD and ACP, respectively, with 1 μL sample loading. The running time was 30–40 min. Gene frequencies for ESD were 0.6809 for ESD*1 and 0.3191 for ESD*2, (∑χ 2 = 0.8586 and P ≥ 0.50); gene frequencies of ACP were 0.2009 for ACP*A, 0.7991 for ACP*B, (∑χ 2 = 1.7892 and P > 0.20), with good agreement between the observed and the expected values.