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Phenotyping of esterase D and acid phosphatase by rapid isoeletric focusing on PhastSystem
Author(s) -
Yaojun Guo,
Lili Huang,
Xuegang Yao,
Zhongyi Yu
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170322
Subject(s) - esterase , acid phosphatase , biochemistry , phosphatase , chemistry , computational biology , biology , enzyme
A rapid isoelectric focusing method on PhastSystem was applied to investigate the distribution of esterase D (ESD) and acid phosphatase (ACP) phenotypes in 152 and 112 unrelated healthy donors, respectively, from the Han population in Beijing. Laboratory‐made gels with a pH range of 5.0–7.0 and 5.0–8.0 were used for analysis of ESD and ACP, respectively, with 1 μL sample loading. The running time was 30–40 min. Gene frequencies for ESD were 0.6809 for ESD*1 and 0.3191 for ESD*2, (∑χ 2 = 0.8586 and P ≥ 0.50); gene frequencies of ACP were 0.2009 for ACP*A, 0.7991 for ACP*B, (∑χ 2 = 1.7892 and P > 0.20), with good agreement between the observed and the expected values.