Sodium chloride in preparative free‐flow cell electrophoresis: Recent developments | Zendy

Bauer Johann | Zendy; Weber Gerhard | Zendy

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Sodium chloride in preparative free‐flow cell electrophoresis: Recent developments

Author(s) -

Bauer Johann,

Weber Gerhard

Publication year - 1996

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150170319

Subject(s) - bromophenol blue , electrophoresis , buffer (optical fiber) , chromatography , free flow electrophoresis , chemistry , sodium , laminar flow , free flow , electrode , suspension (topology) , analytical chemistry (journal) , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , computer science , telecommunications , physics , mathematics , homotopy , pure mathematics , thermodynamics , organic chemistry , mechanics , enzyme

Three buffer systems for free‐flow electrophoresis have been designed, which proved useful for performing cell electrophoresis in the presence of 50 m M NaCl. Each system consists of one central cell suspension buffer with 50 m M NaCl, two margin buffers, and two electrode buffers. With the aid of a bromophenol blue/maxilon blue accumulation test the various buffers were adjusted to ensure a laminar flow and remain unchanged on their way through an electrophoresis chamber.

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