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High‐speed separation of subnanomolar concentrations of noradrenaline and dopamine using capillary zone electrophoresis with laser‐induced fluorescence detection
Author(s) -
Bert Lionel,
Robert Frédéric,
Denoroy Luc,
Renaud Bernard
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170318
Subject(s) - capillary electrophoresis , laser induced fluorescence , fluorescence , chromatography , dopamine , catecholamine , chemistry , resolution (logic) , detection limit , capillary action , analytical chemistry (journal) , materials science , biology , optics , endocrinology , physics , artificial intelligence , computer science , composite material
Capillary zone electrophoresis with laser‐induced fluorescence detection has been shown to give rapid separations with high resolution and sensitivity. These advantages are documented for catecholamines analysis in the present work, which shows that separation of subnanomolar concentrations of dopamine and noradrenaline (detection limits of 8.6 × 10 −11 M, corresponding to a detected amount of 143 zeptomoles of each catecholamine) can be performed in 82 s with an efficiency of several million theoretical plates.