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Soldium dodecyl sulphate‐polyacrylamide gel electrophoresis of human parotid salivary proteins: Comparison of dansylation, Coomassie Blue R‐250 and silver detection methods
Author(s) -
Beeley Josie A.,
Newman Frances,
Wilson Paul H. R.,
Shimmin Ian C.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170314
Subject(s) - chromatography , silver stain , polyacrylamide gel electrophoresis , chemistry , coomassie brilliant blue , transillumination , polyacrylamide , gel electrophoresis of proteins , staining , gel electrophoresis , biochemistry , biology , microbiology and biotechnology , enzyme , medicine , genetics , pathology , polymer chemistry
Abstract Detection of human parotid salivary proteins by dansylation and UV‐transillumination after sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) has been compared with Coomassie Blue R‐250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline‐rich proteins (PRPs).