Preparative isoelectric focusing and Joule effect: A purification cell that contains a heat exchanger | Zendy

Araque Arturo | Zendy; Jaugey Jean | Zendy; Javet Philippe | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Preparative isoelectric focusing and Joule effect: A purification cell that contains a heat exchanger

Author(s) -

Araque Arturo,

Jaugey Jean,

Javet Philippe

Publication year - 1996

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150170127

Subject(s) - isoelectric focusing , myoglobin , isoelectric point , chromatography , chemistry , bovine serum albumin , hemoglobin , membrane , albumin , protein purification , biochemistry , enzyme

Reproducibility in protein purification by preparative isoelectric focusing depends greatly on temperature control during the separation process. A preparative apparatus is described, including a heat exchanger between compartments with isoelectric membranes. The selectivity of the isoelectric membranes was optimized as a function of isoelectric points of the separated proteins. At 2500 V and 60 W, 0.3 g of horse heart myoglobin from 0.2 g of whale skeletal muscle myoglobin could be separated in 1 h. At a total load of 2 g protein, 97% of bovine hemoglobin (2% initial concentration) was purified from bovine serum albumin (0.15%).

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research