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Ultrafast high resolution separation of large DNA fragments by pulsed‐field capillary electrophoresis
Author(s) -
Kim Yongseong,
Morris Michael D.
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170125
Subject(s) - capillary electrophoresis , high resolution , ultrashort pulse , resolution (logic) , separation (statistics) , electrophoresis , fragment (logic) , chromatography , pulsed field gel electrophoresis , chemistry , analytical chemistry (journal) , optics , physics , computer science , laser , remote sensing , artificial intelligence , geology , biochemistry , machine learning , genotype , gene , programming language
Pulsed‐field capillary electrophoresis (PFCE) in buffers containing ultradilute polymer solutions in used to separate long chain dsDNA in less than 4 min. Separations are shown to work with chain lengths below 10 kbp and greater than 1.5 Mbp. Several pulse protocols have been examined. If running time is to be minimized, a field inversion with higher peak amplitude in the forward direction than in the reverse, but with equal pulse durations, provides the best resolution. Other protocols can provide higher resolution, but only with longer resolution. Other protocols can provide higher resolution, but only with longer running times.