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Analysis of heparin‐like pharmaceuticals by capillary zone electrophoresis and isotachophoresis
Author(s) -
Malá Zdeňka,
Křváková Ludmila,
Boček Petr
Publication year - 1996
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150170121
Subject(s) - isotachophoresis , capillary electrophoresis , chemistry , analyte , chromatography , electrolyte , electrophoresis , capillary electrochromatography , capillary action , impurity , organic chemistry , materials science , electrode , composite material
Synthetic sulfated bis‐lactobionic acid amides are important heparin‐like pharmaceuticals. The synthesis of these compounds yields molecules differing in the number of sulfate groups, and during the isolation procedure, the required species may partially decompose or take in some impurities. This article shows that capillary zone electrophoresis may serve well as an expedient method for the analysis of the above‐mentioned pharmaceuticals. Complex‐forming equilibria between the analytes and bivalent cations present in the background electrolyte bring selectivity necessary for the separation, and the detection at various wavelengths serves as an aid in the characterization of admixtures, decomposition products, and impurities. Capillary isotachophoresis may also be used for the analysis of these species, bringing about the potential of micropreparation of individual compounds and opening the chance for continuous free‐flow electrophoresis.