Premium
Fused‐silica capillaries with surface‐bound dextran layer crosslinked with diepoxypolyethylene glycol for capillary electrophoresis of biological substances at reduced electroosmotic flow
Author(s) -
Mechref Yehia,
Rassi Ziad El
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160199
Subject(s) - dextran , chemistry , capillary electrophoresis , capillary action , peg ratio , covalent bond , electrophoresis , polyethylene glycol , chromatography , aqueous solution , surface modification , monosaccharide , chemical engineering , coating , organic chemistry , materials science , finance , engineering , economics , composite material
This report is concerned with the introduction of novel surface modification involving the covalent attachment of branched, high molecular weight dextrans and subsequent crosslinking with polyether chains on the inner surface of fused silica capillaries with the aim of producing hydrophilic capillaries with reduced electroosmotic flow (EOF). Three different molecular mass dextrans, namely 45, 71 or 150 kDa were covalently attached to the capillary surface, and subsequently crosslinked with diepoxypolyethylene glycol (PEG). This chemistry produced stable coating over a wide range of conditions including high and low pH aqueous solutions. Moreover, the various dextran‐PEG‐coated capillaries exhibited reduced electroosmotic flow. These features of the dextran‐PEG coatings were useful for the separations of basic and acidic proteins, and provided high resolution separation for closely related acidic monosaccharides and sialooligosaccharides Presented as a poster at Electrophoresis Forum '94, Munich, Germany, October 24–26, 1994 .