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Effect of buffer composition on the migration order and separation of histone H1 subtypes
Author(s) -
Lindner Herbert,
Helliger Wilfried,
Sarg Bettina,
Meraner Christoph
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160197
Subject(s) - chemistry , capillary electrophoresis , chromatography , histone , electrophoresis , buffer (optical fiber) , selectivity , linker , phosphate buffered saline , phosphate , histone h1 , electrochromatography , perchlorate , composition (language) , elution , dna , capillary electrochromatography , biochemistry , ion , organic chemistry , telecommunications , linguistics , philosophy , computer science , operating system , catalysis
The effects of different buffer concentrations and compositions on the elution order and separation of H1 histone subtypes and their phosphorylated modifications isolated from several species was studied using high‐performance capillary electrophoresis (CE). Various cations and anions were tested in an untreated silica capillary and low pH buffers, in the presence of the dynamic coating agent hydroxypropylmethyl cellulose. It was found that the cations and anions of buffers have a remarkable influence on both the efficiency and the selectivity of protein separations. A triethylammonium methanephosphonate system proved efficacious for the separation of rat histone subtype H1c from H1e and a perchlorate/triethylammonium phosphate system for the analysis of chicken and mouse linker histones. CE provides an attractive alternative to high‐performance liquid chromatography and conventional gel electrophoresis.