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Effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis: Theoretical calculation and experimental evaluation
Author(s) -
Cifuentes Alejandro,
Poppe Hans
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160185
Subject(s) - electropherogram , capillary electrophoresis , ionic strength , peptide , electrophoresis , chemistry , chromatography , buffer (optical fiber) , buffer solution , capillary action , analytical chemistry (journal) , range (aeronautics) , materials science , computer science , biochemistry , aqueous solution , organic chemistry , telecommunications , composite material
The effect of pH and ionic strength of running buffer on peptide behavior in capillary electrophoresis (CE) is studied. A system for predictions of peptide migration in CE (SPPMCE) developed in our laboratory has been tested in a wide range of pH and buffer concentrations. The SPPMCE consists of a computer program for calculating peptide p K a values, an equation which relates peptide structures to their electrophoretic mobilities and a coupled computer program for the prediction of electropherograms. More than 25 different buffers have been employed, covering a pH range of 2–11 and a concentration range of 5–100 m M . Results from experiments are compared with the theoretical predictions. Good agreement is observed, which confirms the utility of the SPPMCE and allows fast and easy optimization of peptide separations in CE, with nothing more than the amino acid sequence of the linear peptide as the input.