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Analysis of underivatized amino acids by capillary electrophoresis using constant potential amperometric detection
Author(s) -
Guo Yong,
Colón Luis A.,
Dadoo Rajeev,
Zare Richard N.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160181
Subject(s) - capillary electrophoresis , amperometry , chromatography , chemistry , microelectrode , detection limit , electrophoresis , amino acid , capillary action , hydrolysis , linear range , electrode , analytical chemistry (journal) , electrochemistry , materials science , biochemistry , composite material
A mixture of native (underivatized) amino acids is separated by capillary electrophoresis under alkaline conditions (pH ≈ 12) and amperometrically detected with a copper‐disk microelectrode. A simple design facilitates capillary‐electrode alignment without the need for micropositioning equipment. The limits of detection for the amino acids are in the low μm concentration range, and the signal response is linear over 2–3 orders of magnitude. This procedure is applied to analyze the amino acid hydrolysis products from cytochrome c.