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Microchip electrophoresis with sample stacking
Author(s) -
Jacobson Stephen C.,
Ramsey J. Michael
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160179
Subject(s) - stacking , reproducibility , chromatography , analytical chemistry (journal) , materials science , capillary electrophoresis , buffer (optical fiber) , electrophoresis , etching (microfabrication) , quartz , substrate (aquarium) , chemistry , layer (electronics) , nanotechnology , composite material , telecommunications , oceanography , organic chemistry , geology , computer science
A fused quartz microchip with a serpentine column geometry is fabricated to perform rapid microchip electrophoresis of dansylated amino acids. A 67 mm separation column is constructed in a 7 × 10 mm area on a quartz substrate using standard photolithographic, etching and deposition techniques. Buffer and sample flows within the channel manifold are precisely controlled through potentials applied to the reservoirs. To enhance the detection limits, a stacking injection technique is used to concentrate the sample at the inlet of the separation column. The stacked injections exhibit high reproducibility (2.1% relative standard deviation in peak area). Using a separation length of 67 mm and a separation field strength of 1100 V/cm, separations are performed in ⩽ 15 s generating ≈ 40 000 theoretical plates.