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Studies on the procedure for the construction of cellular protein databases employing micro two‐dimensional electrophoresis: An HL‐60 protein database
Author(s) -
Manabe Takashi,
Yamamoto Hideko,
Kawai Makoto
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160168
Subject(s) - database , electrophoresis , chemistry , computer science , chromatography
The procedure to construct a cellular protein database employing a micro two‐dimensional electrophoresis (2‐DE) technique was studied. Protein information on human cultured leukemic cell HL‐60 was collected as an HL‐60 protein database, which consists of a 2‐DE polypeptide map of 670 recognized spots with spot numbering and information on isoelectric point (p I ), molecular mass, and percent integrated density. The procedure employed in this report will be applicable for the comparative studies of cellular proteins and will help to construct comprehensive cellular protein databases.