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Electrophoretic analysis of bile proteins from patients with and without gallstones
Author(s) -
Ota Yukiko,
Ido Kenichi,
Kimura Ken,
Fukui Emiko,
Ikemoto Shigenori
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160167
Subject(s) - gallstones , chemistry , chromatography , polyacrylamide gel electrophoresis , gel electrophoresis , sodium dodecyl sulfate , electrophoresis , concanavalin a , molecular mass , biochemistry , medicine , in vitro , enzyme
Abstract Bile proteins from 82 cases with various types of gallstones (pure cholesterol stones, cholesterol stones with a small amount of other substances, mixed stones and pigment stones) and 9 controls without gallstones were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). A unique bile protein with a molecular mass of 32 kDa was identified. The 32 kDa protein could be stained on immunoblotting membranes using immunoperoxidase with concanavalin A. The 32 kDa protein was found in all controls but only in a portion (22.2–36.4%) of the patients with gallstones of various types. Following electrophoretic elution from the SDS‐PAGE gels, the 32 kDa protein was analyzed by reversed phase – high‐performance liquid chromatography, yielding three peaks for controls and only two peaks for patients with gallstones.