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Hydrolytically stable cellulose‐derivative coatings for capillary electrophoresis of peptides, proteins and glycoconjugates
Author(s) -
Huang Mingxian,
Plocek Jiri,
Novotny Milos V.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160166
Subject(s) - glycoconjugate , capillary electrophoresis , derivative (finance) , cellulose , chromatography , chemistry , electrophoresis , affinity electrophoresis , biochemistry , enzyme , affinity chromatography , financial economics , economics
A method for the preparation of versatile, hydrolytically stable coatings of fused silica capillaries is described. First, the HNO 3 ‐pretreated silica surface is reacted with 7‐oct‐1‐enyltrimethoxysilane to form a stable self‐assembled alkylsilane monolayer. Secondly, the copolymer of hydroxypropylcellulose and 2‐hydroxyethyl methacrylate is statically coated onto a hydrophobized wall. This allows the formation of a highly crosslinked copolymer layer which is tightly bound to the wall surface. Hydrolytical stability in the pH range of 2–10 for several weeks has been demonstrated. The coating generates a low electroosmotic flow at alkaline pH, which can be easily masked by using special buffers. The versatility of this column technology is documented by separations of peptides, glycoproteins and derivatized oligosaccharides.