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On‐line capillary electrophoresis‐electrospray ionization mass spectrometry of nucleotides
Author(s) -
Zhao Zhongxi Zack,
Wahl Jon H.,
Udseth Harold R.,
Hofstadler Steven A.,
Fuciarelli Alfred F.,
Smith Richard D.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160165
Subject(s) - electrospray ionization , capillary electrophoresis , capillary electrophoresis–mass spectrometry , mass spectrometry , chromatography , chemistry , electrospray , extractive electrospray ionization , protein mass spectrometry , nucleotide , electrophoresis , top down proteomics , analytical chemistry (journal) , biochemistry , gene
Capillary zone electrophoresis (CZE) and reversed anionic capillary isotachophoresis (CITP) conditions have been developed for the separation of mixtures comprised of monophosphate nucleosides, pyridine and flavin dinucleotides, and monophosphate dinucleosides. Results for the on‐line coupling of CZE and CITP with electrospray ionization mass spectrometry (MS) are presented. CITP‐tandem MS is utilized to provide both molecular weight and structural information of monophosphate dinucleosides. The fragmentation pattern of dinucleotides in the low collision energy range is described. The resulting mass spectra are readily interpreted in terms of dinucleotide structures. These results demonstrate the new capability for applications for the study of DNA and RNA.