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Sequence dependent migration behavior of double‐stranded DNA in capillary electrophoresis
Author(s) -
Berka Jan,
Pariat Yvan F.,
Müller Odilo,
Hebenbrock Kirstin,
Heiger David N.,
Foret František,
Karger Barry L.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160164
Subject(s) - capillary electrophoresis , dna , electrophoresis , electrolyte , chemistry , electric field , intercalation (chemistry) , analytical chemistry (journal) , chromatography , gel electrophoresis , polyelectrolyte , polyacrylamide , polymer , biochemistry , inorganic chemistry , polymer chemistry , organic chemistry , physics , electrode , quantum mechanics
Capillary electrophoresis (CE) with a replaceable linear polyacrylamide (LPA) sieving matrix was used to examine sequence‐dependent migration of double‐stranded DNA fragments. It has been found that DNA conformational effects were significant under high electric field separations, especially using high resolution matrices. Compared to linear DNA‐ladder standards, both anomalously slow and rapid DNA fragments were observed, with the degree of anomalous migration depending on the electric field strength, polymer concentration, column temperature, and background electrolyte (denaturants, sodium and magnesium ions, DNA‐intercalating dyes). By selecting a combination of electrophoretic conditions ( e.g. 3% T LPA, elevated capillary temperature, lower electric field strength and addition of DNA intercalating dyes), molecular weight dependent separations were closely restored Presented in part at HPCE '94, San Diego, CA, February, 1994. .