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Rapid and effective Western blotting of histones from acid‐urea‐Triton and sodium docecyl sulfate polyacrylamide gels: Two different approaches depending on the subsequent qualitative or quantitative analysis
Author(s) -
Thiriet Christophe,
Albert Philippe
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160161
Subject(s) - nitrocellulose , chemistry , sodium dodecyl sulfate , polyacrylamide , urea , chromatography , membrane , acetic acid , electrophoresis , sodium , biochemistry , polymer chemistry , organic chemistry
An improved method for the electrophoretic transfer of histones from sodium dodecyl sulfate (SDS) and acetic acid‐urea‐Triton X‐100 (AUT) polyacrylamide gels onto nitrocellulose membranes is described. In the case of SDS‐gels, it was not essential to equilibrate them before transfer while for the AUT‐gels, an equilibration step is essential to prevent the interference of Triton X‐100 with the binding of histones to nitrocellulose. Transfer efficiency was different for different histone classes. Two procedures were developed: (i) one suitable for qualitative studies, and (ii) another for quantitative transfer.