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A highly discriminating octoplex short tandem repeat polymerase chain reaction system suitable for human individual identification
Author(s) -
Oldroyd Nicola J.,
Urquhart Andrew J.,
Kimpton Colin P.,
Millican Emma S.,
Watson Stephanie K.,
Downes Teresa,
Gill Peter D.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160155
Subject(s) - polymerase chain reaction , biology , multiplex , microsatellite , genetics , multiplex polymerase chain reaction , tandem , amelogenin , allele , computational biology , microbiology and biotechnology , gene , materials science , composite material
Through the use of fluorescence‐based polymerase chain reaction systems, a highly discriminating multiplex with the potential for individual identification has been developed. The use of multiple dye technology enabling loci with overlapping size ranges to be co‐amplified has enabled us to successfully amplify seven tetranucleotide short tandem repeat loci within a single reaction in a discriminating power in the region of 1 × 10 9 . Three out of the seven loci employed exhibit alleles differing in size by only 2 bp as opposed to the conventional 4 bp, which results in such loci being more powerful in terms of distinguishing between samples, particularly when co‐amplified in this manner. The size ranges of the loci contained within the system are such that windows still exist for the inclusion of additional loci at a later stage, which could increase the discriminating power of the system still further. In addition, further weight and utility is lent to the system through the incorporation of a simple and reliable sex test involving the amplification of a segment of the X–Y homologous gene Amelogenin.