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Uses of digital image analysis in electrophoresis
Author(s) -
Horgan Graham W.,
Glasbey Chris A.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160149
Subject(s) - digital image analysis , electrophoresis , digital image , computer science , image (mathematics) , chromatography , chemistry , artificial intelligence , computer vision , image processing
The information in electrophoretic gels can be extracted visually, or by using specialised computer hardware and software which treat the gels as digital images. We discuss issues which arise in applying digital image analysis to electrophoresis, and comment on the range of computer packages available. We also illustrate how image analysis can make gel interpretation easier and more reliable. Methods covered include gel registration, and warping, mathematical morphology and deconvolution to sharpen images.