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A single gel analysis of 575 dominant and codominant restriction landmark genomic scanning loci in mice interspecific backcross progeny
Author(s) -
Okuizumi Hisato,
Okazaki Yasushi,
Ohsumi Tomoya,
Hanami Takeshi,
Mizuno Yosuke,
Muramatsu Masami,
Hayashizaki Yoshihide,
Plass Christoph,
Chapman Verne M.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160141
Subject(s) - biology , backcrossing , genetics , genome , genome scan , gene mapping , restriction map , gene , microsatellite , chromosome , allele , nucleic acid sequence
We identified 575 new Not I landmarks of C57BL/6(B)‐ and, M. spretus (S)‐specific, dominant and codominant loci which were segregated in B × S interspecific backcrosses (BSS), using the restriction landmark genomic scanning (RLGS) spot mapping method. All of these loci were visualized on a single RLGS profile which was produced with Not I‐ Pvu II‐ Pst I. These landmarks include 250 newly identified S‐specific spots in addition to the previously reported 325 B‐specific spots. The S‐specific spots were identified by reading full or half intensity, based on the property that the spot intensity of the autoradiographic signal reflected the copy number of an end‐labeled restriction landmark. The cumulative map is 1341 cM and it is based upon 985 meiotic events in 72 backcross progeny. This map covers 90% of the total estimated length of the mouse genetic map. This map provides a good tool for the high‐speed genome scanning assay in the mouse genome by a single RLGS gel analysis.