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Capillary isoelectric focusing: A routine method for protein analysis?
Author(s) -
Schwer Christine
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601342
Subject(s) - isoelectric focusing , chromatography , calibration curve , capillary action , reproducibility , resolution (logic) , chemistry , capillary electrophoresis , analytical chemistry (journal) , materials science , computer science , detection limit , artificial intelligence , biochemistry , composite material , enzyme
Although isoelectric focusing (IEF) in the slab gel format is a widely used technique in protein analysis, capillary isoelectric focusing (CIEF) is not yet a method used routinely, despite major advantages. This may be due to the fact that several methods for performing IEF in the capillary can be found in the literature, differing in the manner of mobilizing the focused protein zones for detection. We therefore discuss and compare the three most widely used CIEF techniques – using chemical, pressure, or electroosmotic flow (EOF) mobilization – with respect to linearity of the calibration curve, resolution, and reproducibility.