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Specific staining of glycine N ‐methyltransferase
Author(s) -
Santos Fátima,
Amorim António,
Kömpf Jost
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601312
Subject(s) - isoelectric focusing , sarcosine , glycine , staining , isoelectric point , biochemistry , methyltransferase , glycosylation , protein subunit , microbiology and biotechnology , methylation , biology , chemistry , enzyme , amino acid , genetics , gene
Glycine N ‐methyltransferase from rabbit, human, rat and pig livers was separated by isoelectric focusing and a specific functional staining method was developed through the detection of sarcosine produced from the methylation of glycine. Isozyme patterns obtained in the various species tested differ both in the number of bands and apparent isoelectric points. These differences may explain the contradictory data on the subunit structure and glycosylation status of the enzyme reported so far.