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Genotyping of human deoxyribonuclease I polymorphism by the polymerase chain reaction
Author(s) -
Yasuda Toshihiro,
Nadano Daita,
Tenjo Etsuko,
Takeshita Haruo,
Sawazaki Kazumi,
Nakanaga Masao,
Kishi Koichiro
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601310
Subject(s) - genotyping , polymerase chain reaction , deoxyribonuclease i , biology , microbiology and biotechnology , genetics , variants of pcr , genotype , dna , allele , deoxyribonuclease , polymerase , gene , base sequence
We recently completely elucidated the molecular basis of genetic polymorphism in human deoxyribonuclease I and found it to be controlled by four codominant alleles, DNASE1 *1, *2, *3 and *4. In this paper we describe a novel DNase I‐genotyping system that could be used directly on DNA samples using the polymerase chain reaction (PCR) based on the three nucleotide substitutions underlying the protein polymorphism. The system consists of three independent reactions. Since the substitutions neither suppress nor create any known enzyme recognition site in the DNase I gene, two separate mismatched PCR followed by Xho I digestion methods were introduced to discriminate between the DNASE1 *1 (or *3) and the DNASE1 *2 (or *4) alleles, and to detect the DNASE1 *4 allele. An amplification refractory mutation system was employed to detect DNASE1 *3. A 100% correlation was found between the results of this genotyping method and those obtained by phenotyping using conventional isoelectric focusing. The high sensitivity and specificity of this genotyping method allows us to survey DNase I‐polymorphism in small DNA samples.