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One‐dimensional genome scanning: Identification of the basis of a mouse mutation and identification of genomic changes in ovarian carcinoma
Author(s) -
Brilliant Murray H.,
Gondo Yoichi,
Magliocco Anthony
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150160129
Subject(s) - genome , biology , genetics , genomic dna , human genome , comparative genomic hybridization , southern blot , dna , microbiology and biotechnology , computational biology , gene
We have developed a simple one‐dimensional electrophoretic method, genome scanning, that can be used to identify large‐scale genomic differences between two or more DNA samples. Genome scanning is especially useful in the detection of genetic amplifications, deletions, and rearrangements. The assay is essentially a high‐resolution Southern analysis, comparing equivalent amounts of genomic DNA samples that are variant for a given trait. The Southern blots are hybridized to a probe sequence derived from a medium copy number repetitive element (1000–2000 copies per haploid genome) naturally dispersed throughout the genome. The hybridization pattern that results is complex and consists of hundreds of bands. If the DNA samples are otherwise equivalent, a net difference in hybridization intensity between homologous bands of different samples indicates a genetic change. In this report, we discuss the origin of the method, its premise, and review its application to mouse mutational analysis and to human cancer research (a more detailed discussion of the theory is presented elsewhere in this issue; Y. Gondo and M. H. Brilliant, Electrophoresis 1995, 16 , 174–178).