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DNA fingerprinting of Brassica juncea cultivars using microsatellite probes
Author(s) -
Bhatia Sabhyata,
Das Sandip,
Jain Ajay,
Lakshmikumaran Malathi
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601289
Subject(s) - rapd , dna profiling , cultivar , biology , microsatellite , germplasm , brassica , ecori , dna , genetics , botany , genetic diversity , gene , restriction enzyme , allele , population , demography , sociology
The genetic variability in the Brassica juncea cultivars was detected by employing in‐gel hybridization of restricted DNA to simple repetitive sequences such as (GATA) 4 , (GACA) 4 and (CAC) 5 . The most informative probe/enzyme combination was (GATA) 4 / Eco RI, yielding highly polymorphic fingerprint patterns for the B. juncea cultivars. This technique was found to be dependable for establishing the variety specific patterns for most of the cultivars studied, a prerequisite for germplasm preservation. The results of the present study were compared with those reported in our earlier study in which random amplification of polymorphic DNA (RAPD) was used for assessing the genetic variability in the B. juncea cultivars.