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DNA profiling of banana and plantain cultivars using random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers
Author(s) -
Bhat K. Venkataramana,
Jarret Robert L.,
Rana Rai Singh
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601287
Subject(s) - rapd , biology , restriction fragment length polymorphism , genomic dna , genetics , genome , germplasm , polymerase chain reaction , ecori , southern blot , dna profiling , genetic marker , hindiii , dna , restriction enzyme , gene , botany , genetic diversity , population , demography , sociology
Polymerase chain reaction (PCR) amplification of genomic DNA from 57 Musa cultivars with 60 random 10‐mer primers generated 605 polymorphic amplification products which were useful in unambiguous cultivar identifications. Unweighted pair‐group method analysis of this data grouped the cultivars into specific clusters depending on their genomic similarities. The diploid ancestral species of cultivated banana and plantains, namely Musa acuminata ssp malaccensis , an A genome donor and M. balbisiana , a B genome donor, were farthest apart from each other in the phenogram. The edible fruit yielding cultivars with the genomic constitutions AA, AAA, AB, AAB, ABB and ABBB grouped in different clusters according to overall genetic homologies. The restriction fragment length polymorphisms (RFLPs) prevalent among the cultivars were studied by hybridizations of 19 random genomic clones to blots of Hin dIII, Eco RI and Msp I digests. Cluster analysis of these data on 107 polymorphic alleles resulted in a phenogram comparable to the one obtained with random amplified polymorphic DNA (RAPD) analysis. Two multilocus probes useful in distinguishing all the 57 cultivars analyzed were also identified. The A and B types of cytoplasms in the cultivars were further distinguished by hybridization of heterologous chloroplast DNA probes. Results showed that use of different kinds of molecular markers in gene banks is essential for characterization and classification of germplasm collections.