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Allele frequency distribution for the variable number of tandem repeat locus D10S28 in Tamil Nadu (South India) population
Author(s) -
Pandian S. Karutha,
Kumar Subbaiah,
Krishnan Manikkam,
Dharmalingam Kuppamuthu,
Damodaran Chenniappan
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601280
Subject(s) - tamil , loss of heterozygosity , locus (genetics) , microsatellite , allele frequency , allele , population , variable number tandem repeat , tandem repeat , biology , genetics , veterinary medicine , demography , gene , medicine , sociology , philosophy , linguistics , genome
Allele frequencies were determined in unrelated individuals of Tamil speaking population from the Madras City (Tamil Nadu, South India) area for the polymorphic DNA locus D10S28 using the probe TBQ7. Membranes hybridized with the probe YNH24 were subjected to deprobing and were subsequently hybridized with random priming – labeled, purified inserts of TBQ7. The sizes of the fragments were grouped to 100 bp as well as to arbitrary fixed bins (Federal Bureau of Investigation/Royal Canadian Mounted Police). There were 14 bins in the latter with the most common bin being 11 (1789–1924 bp) with a frequency of 9.8%. We observed a heterozygosity of 92% comparable to Caucasian populations. The data presented here can be used as the basis for utilizing this variable number of tandem repeats (VNTR) DNA marker for paternity determinations and forensic investigations.