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DNA fingerprinting in silkworm Bombyx mori using banded krait minor satellite DNA‐derived probe
Author(s) -
Nagaraju Javaregowda,
Sharma Abhay,
Sethuraman Balakathiresan N.,
Rao G. V.,
Singh Lalji
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601271
Subject(s) - biology , satellite dna , bombyx mori , dna profiling , genomic dna , dna , genetics , microbiology and biotechnology , gene , chromosome
The genomic DNA from thirteen different ecotypes and inbred lines of silkworm, Bombyx mori , were analyzed by digesting with Bst NI and Hin fI restriction enzymes followed by hybridization with banded krait minor satellite DNA (Bkm)–2(8) minisatellite probe. The DNA fingerprinting revealed 9–31 discrete intense bands, some of which were ecotype/inbred line‐specific. Individual specific DNA fingerprints in two representative genotypes and their F 1 hybrid offspring were also obtained. Individuals of a given parental line showed very similar profiles and the hybrid offspring showed the combined profile of both parents. The presence of bands specific to diapausing and non‐diapausing strains and to particular genotypes indicate their potential use for marker‐assisted breeding and varietal identification.