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Further study on the use of uncharged β‐cyclodextrin polymer in capillary electrophoresis: Enantiomeric separation of some α‐hydroxy acids
Author(s) -
Fanali Salvatore,
Aturki Zeineb
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601249
Subject(s) - capillary electrophoresis , chemistry , enantiomer , cyclodextrin , mandelic acid , electrolyte , chromatography , polymer , resolution (logic) , electrophoresis , organic chemistry , electrode , artificial intelligence , computer science
Uncharged β‐cyclodextrin polymer was used as chiral selector for the enantiomeric separation of some α‐hydroxy acids by capillary electrophoresis. Complexation and enantiomeric resolution of mandelic acid, m ‐hydroxy and p ‐hydroxymandelic acid, 3,4‐dihydroxymandelic acid, as well as 2‐ and 3‐phenyllactic acid were studied, changing the concentration of the β‐cyclodextrin polymer added to the background electrolyte at different pH in the range of 4.5–7. Furthermore, the effects of the concentration of the background electrolyte, column temperature, and applied voltage on chiral resolution were also examined. The best enantiomeric separations were obtained using a background electrolyte at pH 6 containing 100 mg/mL of β‐cyclodextrin polymer.