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Effect of organic modifier concentrations on electrokinetic migrations in micellar electrokinetic chromatography
Author(s) -
Chen g,
Terabe Shigeru,
Nakagawa Terumichi
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601241
Subject(s) - micellar electrokinetic chromatography , electrokinetic phenomena , chemistry , micelle , electrophoresis , sodium dodecyl sulfate , chromatography , phase (matter) , viscosity , analytical chemistry (journal) , organic chemistry , materials science , aqueous solution , composite material
The effect of low concentrations of organic modifiers on the electroosmotic mobility, μ eo , and electrophoretic mobility of the micelle, μ ep, mc , in micellar electrokinetic chromatography (MEKC) with sodium dodecyl sulfate (SDS) solutions was investigated. The results showed that μ eo was mainly influenced by the viscosity of the running solution. A linear relationship between μ eo and the concentration of the organic modifier was found in MEKC. A newly defined parameter, the mobility ratio, R m, mc , which is the ratio of the electro‐phoretic mobility of the micelle to the electroosmotic mobility, and which characterizes the surface charge density of the micelle in MEKC system with organic modifiers, was introduced. A linear relationship between R m, mc and the organic modifier concentration was observed. The parameter R m, mc was found to be useful to investigate the changes in the micellar phase with the addition of organic modifier. The measured μ ep, mc values showed different and interesting characteristics among different organic modifiers.