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Influence of electrolyte composition on the effective electric field strength in capillary zone electrophoresis
Author(s) -
Jumppanen Juho H.,
Riekkola MarjaLiisa
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601238
Subject(s) - capillary electrophoresis , electrolyte , electrophoresis , electric field , composition (language) , analytical chemistry (journal) , capillary action , chemistry , capillary electrochromatography , chromatography , materials science , composite material , electrode , physics , quantum mechanics , linguistics , philosophy
In capillary zone electrophoresis the electrophoretic velocity of an ion decreases as the concentration of the background electrolyte solution is increased. This is caused by changes in the electrophoretic mobility of the ion (μ ep ) as well as by changes in the net force affecting it, namely the effective electric field strength ( E eff ). The electrophoretic mobility of an ion is altered through changes in absolute viscosity of the electrolyte solution and changes in the solvated size of the ion. E eff is altered mainly by changes in the magnitude of the charge asymmetry effect and the electrophoretic effect, both of which retard the motion of ions. In this study, the three‐marker technique was used to study the effect of background electrolyte concentration (0.02–0.08 M 3‐[cyclohexylamino]‐1‐propanesulfonic acid and counter ion (Li, Na, K, and Cs) on E eff . It was found that the concentration of the background electrolyte markedly affects E eff and that E eff approaches E as the concentration of the background electrolyte approaches zero. The counter ion had a minor effect on E eff : as the size of the hydrated radius of the counter ion increased, E eff decreased. The three‐marker technique proved to be efficient for such determinations.