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DNA separation with field inversion capillary electrophoresis
Author(s) -
Heller Christoph,
Pakleza Christophe,
Viovy Jean Louis
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601235
Subject(s) - capillary electrophoresis , electrophoresis , electric field , chromatography , analytical chemistry (journal) , dna , field (mathematics) , materials science , pulsed field gel electrophoresis , chemistry , physics , biochemistry , mathematics , quantum mechanics , pure mathematics , genotype , gene
We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double‐stranded DNA in the range of 1–50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.