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Manipulation of a single cell with microcapillary tubing based on its electrophoretic mobility
Author(s) -
Kitagawa Shinya,
Kawaura Chiyo,
Hashimoto Osamu,
Takahashi Tsutomu,
Naoi Makoto,
Tsuda Takao
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601225
Subject(s) - capillary action , electrophoresis , electric field , voltage , dielectrophoresis , trapping , materials science , chemistry , electro osmosis , flow (mathematics) , microfluidics , analytical chemistry (journal) , chromatography , mechanics , nanotechnology , composite material , electrical engineering , physics , ecology , quantum mechanics , biology , engineering
Manipulation of a single cell of spherical shape, approximately 5–10 μm in diameter, was performed with capillary tubing and an electrostatic field. A single cell migrates with its electrophoretic mobility into capillary tubing against the flow of electroosmosis coming out of the capillary. After trapping the cell in the capillary, it is pulled out into the other microreservoir with the application of a reverse electric voltage. When we apply a negative voltage to the microreservoir itself, the cell in it can keep floating for a relatively long period due to electrostatic repulsion. The electrophoretic mobility of a single cell is also estimated.