Premium
Phenotyping of apolipoprotein E using immobilized pH gradient gels for one‐dimensional and two‐dimensional separations
Author(s) -
Golaz Olivier,
Sanchez JeanCharles,
James Richard W.,
Hochstrasser Denis F.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601196
Subject(s) - apolipoprotein b , apolipoprotein e , lipoprotein , phenotype , chemistry , ldl receptor , cholesterol , plasma lipoprotein , biochemistry , disease , medicine , gene
Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)‐cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E‐2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeld‐Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time‐consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods.