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Sample preparation of human tumors prior to two‐dimensional electrophoresis of proteins
Author(s) -
Franzén Bo,
Hirano Takashi,
Okuzawa Ken,
Uryu Kazuto,
Alaiya Ayodele A.,
Linder Stig,
Auer Gert
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601184
Subject(s) - sample preparation , reproducibility , chromatography , electrophoresis , stromal cell , sample (material) , resolution (logic) , high resolution , chemistry , computer science , biology , cancer research , artificial intelligence , remote sensing , geology
Sample preparation procedures are critical for obtaining high quality two‐dimensional electrophoresis (2‐DE) separations from clinical tumor materials. In this communication, we describe procedures which we have found satisfactory for preparing various tumor samples for 2‐DE. By these procedures, tumor cells are enriched from serum proteins and contaminating stromal cells. Tumors can be prepared with good reproducibility to obtain high resolution using these techniques.