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Direct detection of β‐1,3‐glucanase in plant extracts by polyacrylamide gel electrophoresis
Author(s) -
Kalix Siegfried,
Buchenauer Heinrich
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601170
Subject(s) - glucanase , polyacrylamide gel electrophoresis , chromatography , chemistry , gel electrophoresis , two dimensional gel electrophoresis , electrophoresis , biochemistry , proteomics , enzyme , gene
By using carboxymethyl (CM)‐curdlan, a polysaccharide linked with the dye Remazol Brilliant Blue (RBB) as a substrate in polyacrylamide gels, the β‐1,3‐glucanase in plant extracts can be detected directly by native polyacrylamide gel electrophoresis. In contrast to the usually used procedures for the detection of glucanases, e.g. , colorimetric assay, overlay technique, enzyme activity staining using laminarin as a substrate, this method is rapid and allows both the determination of the activity and the location of the relative position of the multiple forms of β‐1,3‐glucanases.