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A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels
Author(s) -
Swain Marianne,
Ross Neil W.
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601159
Subject(s) - silver stain , sodium dodecyl sulfate , staining , polyacrylamide , chromatography , chemistry , acetic acid , polyacrylamide gel electrophoresis , glutaraldehyde , stain , gel electrophoresis , sodium , resolution (logic) , gel electrophoresis of proteins , biochemistry , microbiology and biotechnology , biology , enzyme , polymer chemistry , organic chemistry , genetics , artificial intelligence , computer science
A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/acetic acid and prior to glutaraldehyde cross‐linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.