Premium
The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis
Author(s) -
Hilbert Friederike,
Mayr Burkhard,
Lackner Fritz,
Bauer Friedrich
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601152
Subject(s) - silver stain , nucleic acid , staining , polyacrylamide gel electrophoresis , polyacrylamide , polymerase , restriction enzyme , gel electrophoresis , gel electrophoresis of nucleic acids , dna , bovine serum albumin , microbiology and biotechnology , electrophoresis , chemistry , biochemistry , polymerase chain reaction , nucleic acid quantitation , enzyme , biology , gene , genetics
The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12–20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, Taq ‐DNA polymerase) interfere with silver staining of nucleic acids.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom