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The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis
Author(s) -
Hilbert Friederike,
Mayr Burkhard,
Lackner Fritz,
Bauer Friedrich
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601152
Subject(s) - silver stain , nucleic acid , staining , polyacrylamide gel electrophoresis , polyacrylamide , polymerase , restriction enzyme , gel electrophoresis , gel electrophoresis of nucleic acids , dna , bovine serum albumin , microbiology and biotechnology , electrophoresis , chemistry , biochemistry , polymerase chain reaction , nucleic acid quantitation , enzyme , biology , gene , genetics
The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12–20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, Taq ‐DNA polymerase) interfere with silver staining of nucleic acids.