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Genotyping apolipoprotein B signal peptide insertion/deletion: A comparison of three methods
Author(s) -
Glisic Sanja,
Sunjevaric Ivana,
Alavantic Dragan
Publication year - 1995
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.11501601151
Subject(s) - genotyping , ethidium bromide , agarose , microbiology and biotechnology , apolipoprotein b , biology , allele , genotype , signal peptide , polyacrylamide , agarose gel electrophoresis , silver stain , genetics , gene , biochemistry , dna , peptide sequence , cholesterol
Three different ways of detecting insertion/deletion (Ins/Del) genotypes of the apolipoprotein B gene encoding signal peptide were compared. Unequal quantities of the two alleles were observed in 50 Serbian subjects. The low abundance allele was clearly visible only when a modified method of silver staining for polyacrylamide gels was used. This method is more reliable for Ins/Del polymorphism genotyping, avoiding misclassifications by conventional ethidium bromide staining of both agarose and polyacrylamide gels.